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PORCINETRACE PCR KITS

PorcineTrace PCR kits

PorcineTrace PCR kits

 

Description

7FoodPillars PorcineTrace PCR Kit provides a fast, direct, sensitive and reliable detection of DNA extracted from a wide variety of food products in approximately 3 hours.

Intended Use

The PorcineTrace PCR Kit is designed to detect the specific gene for porcine species in food. This kit provides PCR Mastermix with enzyme components and the primer set for rapid testing by conventional PCR assay, as well as the Internal Control (IC) system for reliable results.

Principle Of Test

The Porcine Trace PCR kit employs a multiplex Polymerase Chain Reaction test platform which selectively amplifies 3 amplicons; one of which is used as an External control. The amplicons are specific gene fragments to detect porcine, vertebrate and plant DNA. In principle, this test could also be used to detect traces of meat (vertebrate band); for discerning vegetarians but as it is optimized primarily for detection of porcine DNA; sensitivity for this kind of usage might not be as good as for detection of porcine traces.

Reagents And Materials Supplied

Components PHAL01-050 PHAL01-100
PorcineTace Mastermix 1 tube x 550μl 1 tube x 1100μl
PorcineTrace Primer mix 1 tube x 110μl 1 tube x 220μl
PorcineTrace PCR Water 1 tube x 1000μl 2 tubes x 1000μl
PorcineTrace DNA marker 1 tube x 50μl 1 tube x 100μl
External control DNA, 10ng/μl* 1 tube x 50μl 1 tube x 100μl
Positive Control DNA, 10ng/μl* 1 tube x 50μl 1 tube x 100μl

Storage And Stability

Store all reagents at -20°C. Stable up to the expiry date stated.

Result Interpretation

PorcineTrace

News

Lane/ Case Band visible (bp) Interpretation of result
398 359 (IC) 303 (EC)
1 NA NA NA 100bp DNA Ladder
2 + + + PorcineTrace DNA Marker
3 + + + Porcine specific gene is detected in sample
4 + + Porcine specific gene is detected in sample
5 + Porcine specific gene is NOT detected in sample
6 + Porcine specific gene is NOT detected in sample
7 + Porcine specific gene is NOT detected in sample

 

Valid if EC is not included

Invalid if EC is included